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Arsenic in PDB 1sjz: Arsenate Reductase R60K Mutant +0.4M Arsenite From E. Coli

Enzymatic activity of Arsenate Reductase R60K Mutant +0.4M Arsenite From E. Coli

All present enzymatic activity of Arsenate Reductase R60K Mutant +0.4M Arsenite From E. Coli:
1.20.4.1;

Protein crystallography data

The structure of Arsenate Reductase R60K Mutant +0.4M Arsenite From E. Coli, PDB code: 1sjz was solved by S.Demel, B.F.Edwards, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 20.00 / 1.80
Space group P 61 2 2
Cell size a, b, c (Å), α, β, γ (°) 86.720, 86.720, 116.090, 90.00, 90.00, 120.00
R / Rfree (%) 18.3 / 23.9

Other elements in 1sjz:

The structure of Arsenate Reductase R60K Mutant +0.4M Arsenite From E. Coli also contains other interesting chemical elements:

Caesium (Cs) 3 atoms

Arsenic Binding Sites:

The binding sites of Arsenic atom in the Arsenate Reductase R60K Mutant +0.4M Arsenite From E. Coli (pdb code 1sjz). This binding sites where shown within 5.0 Angstroms radius around Arsenic atom.
In total 2 binding sites of Arsenic where determined in the Arsenate Reductase R60K Mutant +0.4M Arsenite From E. Coli, PDB code: 1sjz:
Jump to Arsenic binding site number: 1; 2;

Arsenic binding site 1 out of 2 in 1sjz

Go back to Arsenic Binding Sites List in 1sjz
Arsenic binding site 1 out of 2 in the Arsenate Reductase R60K Mutant +0.4M Arsenite From E. Coli


Mono view


Stereo pair view

A full contact list of Arsenic with other atoms in the As binding site number 1 of Arsenate Reductase R60K Mutant +0.4M Arsenite From E. Coli within 5.0Å range:
probe atom residue distance (Å) B Occ
A:As12

b:47.3
occ:0.45
AS A:CZ212 0.0 47.3 0.5
O2 A:CZ212 1.8 28.9 0.5
O1 A:CZ212 1.8 26.5 0.5
SG A:CZ212 2.2 8.8 0.5
SG A:CZ212 2.4 17.1 0.6
NH2 A:ARG94 2.9 18.8 0.4
CZ A:ARG94 3.1 27.4 0.4
O1 A:TAS201 3.2 5.0 0.4
CB A:CZ212 3.2 8.2 0.5
CB A:CZ212 3.2 14.1 0.6
NH1 A:ARG94 3.3 21.2 0.4
AS A:TAS201 3.4 35.9 0.4
CA A:CZ212 3.7 19.1 1.0
NH2 A:ARG94 3.8 20.6 0.6
NE A:ARG94 3.8 25.1 0.4
NH1 A:ARG107 4.0 14.6 1.0
NE A:ARG94 4.1 24.9 0.6
N A:GLY13 4.1 14.4 1.0
C A:CZ212 4.2 17.5 1.0
OG1 A:THR14 4.3 17.3 1.0
N A:THR14 4.3 12.1 1.0
CZ A:ARG94 4.4 26.6 0.6
O A:HOH1001 4.4 47.6 1.0
O2 A:TAS201 4.5 37.9 0.4
CB A:THR14 4.5 14.5 1.0
NH2 A:ARG107 4.6 28.3 1.0
O3 A:TAS201 4.7 12.6 0.4
CD A:ARG94 4.7 20.0 0.4
CZ A:ARG107 4.8 19.7 1.0

Arsenic binding site 2 out of 2 in 1sjz

Go back to Arsenic Binding Sites List in 1sjz
Arsenic binding site 2 out of 2 in the Arsenate Reductase R60K Mutant +0.4M Arsenite From E. Coli


Mono view


Stereo pair view

A full contact list of Arsenic with other atoms in the As binding site number 2 of Arsenate Reductase R60K Mutant +0.4M Arsenite From E. Coli within 5.0Å range:
probe atom residue distance (Å) B Occ
A:As201

b:35.9
occ:0.39
AS A:TAS201 0.0 35.9 0.4
O1 A:TAS201 1.8 5.0 0.4
O3 A:TAS201 1.8 12.6 0.4
O2 A:TAS201 1.8 37.9 0.4
AS A:CZ212 3.4 47.3 0.5
N A:GLY13 3.6 14.4 1.0
O1 A:CZ212 3.9 26.5 0.5
O A:HOH1052 4.0 15.5 1.0
O A:HOH1133 4.2 27.7 1.0
CA A:GLY13 4.2 14.1 1.0
N A:THR14 4.5 12.1 1.0
C A:CZ212 4.6 17.5 1.0
O2 A:CZ212 4.7 28.9 0.5
O A:HOH1321 4.7 42.8 1.0
CA A:CZ212 4.8 19.1 1.0
SG A:CZ212 4.8 8.8 0.5
C A:GLY13 4.9 10.7 1.0
SG A:CZ212 5.0 17.1 0.6

Reference:

S.Demel, J.Shi, P.Martin, B.P.Rosen, B.F.Edwards. Arginine 60 in the Arsc Arsenate Reductase of E. Coli Plasmid R773 Determines the Chemical Nature of the Bound As(III) Product. Protein Sci. V. 13 2330 2004.
ISSN: ISSN 0961-8368
PubMed: 15295115
DOI: 10.1110/PS.04787204
Page generated: Sat Dec 12 01:37:19 2020

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