Arsenic in PDB 1tql: Poliovirus Polymerase G1A Mutant

All present enzymatic activity of Poliovirus Polymerase G1A Mutant:
2.7.7.48;

The structure of Poliovirus Polymerase G1A Mutant, PDB code: 1tql was solved by A.A.Thompson, O.B.Peersen, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	30.00 / 2.30
Space group	P 65
Cell size a, b, c (Å), α, β, γ (°)	127.780, 127.780, 113.265, 90.00, 90.00, 120.00
R / Rfree (%)	23.7 / 26.2

The binding sites of Arsenic atom in the Poliovirus Polymerase G1A Mutant (pdb code 1tql). This binding sites where shown within 5.0 Angstroms radius around Arsenic atom.
In total 4 binding sites of Arsenic where determined in the Poliovirus Polymerase G1A Mutant, PDB code: 1tql:
Jump to Arsenic binding site number: 1; 2; 3; 4;

Arsenic binding site 1 out of 4 in the Poliovirus Polymerase G1A Mutant


Mono view


Stereo pair view

A full contact list of Arsenic with other atoms in the As binding site number 1 of Poliovirus Polymerase G1A Mutant within 5.0Å range: probe atom residue distance (Å) B Occ A:As96 b:97.3 occ:1.00 AS A:CAS96 0.0 97.3 1.0 CE2 A:CAS96 2.2 95.0 1.0 CE1 A:CAS96 2.3 95.2 1.0 SG A:CAS96 2.3 91.6 1.0 CB A:CAS96 3.1 86.0 1.0 OD2 A:ASP99 4.2 81.3 1.0 CA A:CAS96 4.6 83.1 1.0 CG A:ASP99 4.7 81.3 1.0 CB A:GLU98 4.8 84.3 1.0

Arsenic binding site 2 out of 4 in the Poliovirus Polymerase G1A Mutant


Mono view


Stereo pair view

A full contact list of Arsenic with other atoms in the As binding site number 2 of Poliovirus Polymerase G1A Mutant within 5.0Å range: probe atom residue distance (Å) B Occ A:As212 b:70.9 occ:1.00 AS A:CAS212 0.0 70.9 1.0 CE2 A:CAS212 2.2 62.4 1.0 CE1 A:CAS212 2.2 67.5 1.0 SG A:CAS212 2.3 50.6 1.0 CB A:CAS212 3.2 51.6 1.0 CD1 A:LEU374 3.5 58.1 1.0 O A:CAS212 3.5 44.4 1.0 O A:ALA209 3.5 48.1 1.0 C A:CAS212 3.8 45.1 1.0 CA A:CAS212 3.8 47.0 1.0 N A:CAS212 4.0 48.0 1.0 CG A:PRO214 4.3 39.3 1.0 CD2 A:TYR326 4.3 55.6 1.0 CD A:PRO214 4.3 39.2 1.0 CE2 A:TYR326 4.3 58.7 1.0 C A:ALA209 4.4 46.5 1.0 O A:HOH520 4.7 46.0 1.0 CG A:LEU374 4.7 55.3 1.0 CD2 A:LEU374 4.7 56.0 1.0 CB A:ALA209 4.7 42.7 1.0 N A:ASP213 4.7 43.0 1.0 N A:GLY211 5.0 47.1 1.0 C A:GLY211 5.0 47.9 1.0

Arsenic binding site 3 out of 4 in the Poliovirus Polymerase G1A Mutant


Mono view


Stereo pair view

A full contact list of Arsenic with other atoms in the As binding site number 3 of Poliovirus Polymerase G1A Mutant within 5.0Å range: probe atom residue distance (Å) B Occ A:As281 b:62.0 occ:1.00 AS A:CAS281 0.0 62.0 1.0 CE2 A:CAS281 2.2 59.1 1.0 SG A:CAS281 2.2 56.1 1.0 CE1 A:CAS281 2.2 59.4 1.0 CB A:CAS281 3.1 55.9 1.0 O A:SER271 3.8 49.2 1.0 CA A:CAS281 3.8 56.3 1.0 CZ2 A:TRP5 4.2 56.0 1.0 C A:SER271 4.2 51.2 1.0 CA A:HIS272 4.2 54.5 1.0 CB A:HIS272 4.4 53.4 1.0 N A:HIS272 4.4 53.1 1.0 C A:CAS281 4.7 54.6 1.0 CG A:HIS272 4.8 53.4 1.0 CE2 A:TRP5 4.8 55.9 1.0 CD2 A:HIS272 4.8 52.4 1.0 N A:CAS281 4.9 58.4 1.0 N A:VAL282 4.9 51.9 1.0 NE1 A:TRP5 4.9 54.7 1.0 CH2 A:TRP5 4.9 58.3 1.0 CD2 A:HIS270 4.9 56.2 1.0

Arsenic binding site 4 out of 4 in the Poliovirus Polymerase G1A Mutant


Mono view


Stereo pair view

A full contact list of Arsenic with other atoms in the As binding site number 4 of Poliovirus Polymerase G1A Mutant within 5.0Å range: probe atom residue distance (Å) B Occ A:As290 b:84.9 occ:1.00 AS A:CAS290 0.0 84.9 1.0 CE2 A:CAS290 2.2 81.2 1.0 CE1 A:CAS290 2.2 84.3 1.0 SG A:CAS290 2.3 75.8 1.0 CB A:CAS290 3.1 69.8 1.0 CD2 A:LEU268 3.7 38.3 1.0 SD A:MET187 3.8 63.8 1.0 CB A:SER179 4.2 67.8 1.0 CA A:CAS290 4.3 67.6 1.0 CD2 A:LEU154 4.4 56.5 1.0 CE A:MET187 4.5 60.5 1.0 CG A:LEU268 4.6 40.8 1.0 OG A:SER179 4.8 68.4 1.0 CB A:ASP183 4.9 61.8 1.0

A.A.Thompson, O.B.Peersen. Structural Basis For Proteolysis-Dependent Activation of the Poliovirus Rna-Dependent Rna Polymerase Embo J. V. 23 3462 2004.
ISSN: ISSN 0261-4189
PubMed: 15306852
DOI: 10.1038/SJ.EMBOJ.7600357