Arsenic in PDB 1zzt: Bovine Enos N368D/V106M Double Mutant with L-N(Omega)- Nitroarginine-(4R)-Amino-L-Proline Amide Bound

All present enzymatic activity of Bovine Enos N368D/V106M Double Mutant with L-N(Omega)- Nitroarginine-(4R)-Amino-L-Proline Amide Bound:
1.14.13.39;

The structure of Bovine Enos N368D/V106M Double Mutant with L-N(Omega)- Nitroarginine-(4R)-Amino-L-Proline Amide Bound, PDB code: 1zzt was solved by H.Li, M.L.Flinspach, J.Igarashi, J.Jamal, W.Yang, J.A.Gomez-Vidal, E.A.Litzinger, R.B.Silverman, T.L.Poulos, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	47.43 / 2.14
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	56.450, 105.740, 155.600, 90.00, 90.00, 90.00
R / Rfree (%)	18.9 / 23.3

The structure of Bovine Enos N368D/V106M Double Mutant with L-N(Omega)- Nitroarginine-(4R)-Amino-L-Proline Amide Bound also contains other interesting chemical elements:

Iron	(Fe)	2 atoms
Zinc	(Zn)	1 atom

The binding sites of Arsenic atom in the Bovine Enos N368D/V106M Double Mutant with L-N(Omega)- Nitroarginine-(4R)-Amino-L-Proline Amide Bound (pdb code 1zzt). This binding sites where shown within 5.0 Angstroms radius around Arsenic atom.
In total 2 binding sites of Arsenic where determined in the Bovine Enos N368D/V106M Double Mutant with L-N(Omega)- Nitroarginine-(4R)-Amino-L-Proline Amide Bound, PDB code: 1zzt:
Jump to Arsenic binding site number: 1; 2;

Arsenic binding site 1 out of 2 in the Bovine Enos N368D/V106M Double Mutant with L-N(Omega)- Nitroarginine-(4R)-Amino-L-Proline Amide Bound


Mono view


Stereo pair view

A full contact list of Arsenic with other atoms in the As binding site number 1 of Bovine Enos N368D/V106M Double Mutant with L-N(Omega)- Nitroarginine-(4R)-Amino-L-Proline Amide Bound within 5.0Å range: probe atom residue distance (Å) B Occ A:As384 b:56.2 occ:1.00 AS A:CAS384 0.0 56.2 1.0 CE2 A:CAS384 1.8 50.0 1.0 CE1 A:CAS384 1.8 52.9 1.0 SG A:CAS384 2.3 43.2 1.0 CB A:CAS384 3.0 37.1 1.0 CA A:CAS384 3.7 36.8 1.0 CE3 A:TRP324 4.0 35.7 1.0 CD2 A:TRP324 4.0 41.1 1.0 CG A:TRP324 4.2 39.6 1.0 CB A:TRP324 4.3 38.8 1.0 CD1 A:LEU328 4.6 49.1 1.0 N A:CAS384 4.7 36.1 1.0 CZ3 A:TRP324 4.7 37.2 1.0 C A:CAS384 4.7 37.8 1.0 O A:CAS384 4.7 38.5 1.0 CE2 A:TRP324 4.8 41.4 1.0 CD1 A:TRP324 4.9 39.1 1.0

Arsenic binding site 2 out of 2 in the Bovine Enos N368D/V106M Double Mutant with L-N(Omega)- Nitroarginine-(4R)-Amino-L-Proline Amide Bound


Mono view


Stereo pair view

A full contact list of Arsenic with other atoms in the As binding site number 2 of Bovine Enos N368D/V106M Double Mutant with L-N(Omega)- Nitroarginine-(4R)-Amino-L-Proline Amide Bound within 5.0Å range: probe atom residue distance (Å) B Occ B:As384 b:67.0 occ:1.00 AS B:CAS384 0.0 67.0 1.0 CE1 B:CAS384 1.8 65.8 1.0 CE2 B:CAS384 1.8 67.3 1.0 SG B:CAS384 2.3 59.5 1.0 CB B:CAS384 3.0 51.6 1.0 CA B:CAS384 3.6 50.8 1.0 CE3 B:TRP324 4.3 59.4 1.0 CD2 B:TRP324 4.6 59.7 1.0 N B:CAS384 4.6 49.6 1.0 CB B:TRP324 4.6 54.9 1.0 C B:CAS384 4.7 51.9 1.0 O B:CAS384 4.7 54.4 1.0 CG B:TRP324 4.8 57.1 1.0 CZ3 B:TRP324 4.9 57.0 1.0 CD2 B:LEU328 5.0 49.8 1.0

H.Li, M.L.Flinspach, J.Igarashi, J.Jamal, W.Yang, E.A.Litzinger, H.Huang, E.P.Erdal, R.B.Silverman, T.L.Poulos. Exploring the Binding Conformations of Bulkier Dipeptide Amide Inhibitors in Constitutive Nitric Oxide Synthases. Biochemistry V. 44 15222 2005.
ISSN: ISSN 0006-2960
PubMed: 16285725
DOI: 10.1021/BI0513610