Arsenic in PDB 4xag: Cycles of Destabilization and Repair Underlie the Evolution of New Enzyme Function

The structure of Cycles of Destabilization and Repair Underlie the Evolution of New Enzyme Function, PDB code: 4xag was solved by C.J.Jackson, E.Campbell, M.Kaltenbach, N.Tokuriki, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	39.41 / 1.60
Space group	P 21 21 2
Cell size a, b, c (Å), α, β, γ (°)	85.886, 86.304, 88.583, 90.00, 90.00, 90.00
R / Rfree (%)	16.4 / 20.2

The structure of Cycles of Destabilization and Repair Underlie the Evolution of New Enzyme Function also contains other interesting chemical elements:

Zinc

(Zn)

4 atoms

The binding sites of Arsenic atom in the Cycles of Destabilization and Repair Underlie the Evolution of New Enzyme Function (pdb code 4xag). This binding sites where shown within 5.0 Angstroms radius around Arsenic atom.
In total only one binding site of Arsenic was determined in the Cycles of Destabilization and Repair Underlie the Evolution of New Enzyme Function, PDB code: 4xag:

Arsenic binding site 1 out of 1 in the Cycles of Destabilization and Repair Underlie the Evolution of New Enzyme Function


Mono view


Stereo pair view

A full contact list of Arsenic with other atoms in the As binding site number 1 of Cycles of Destabilization and Repair Underlie the Evolution of New Enzyme Function within 5.0Å range: probe atom residue distance (Å) B Occ G:As401 b:57.7 occ:0.67 AS G:CAC401 0.0 57.7 0.7 O2 G:CAC401 1.7 47.7 0.7 O1 G:CAC401 1.8 10.5 0.7 C1 G:CAC401 2.0 39.4 0.7 C2 G:CAC401 2.0 24.7 0.7 ZN G:ZN403 3.2 16.6 1.0 O G:HOH699 3.3 31.3 1.0 ZN G:ZN402 3.3 13.8 1.0 O G:HOH669 3.4 40.8 1.0 O G:HOH688 3.6 41.0 1.0 OD2 G:ASP301 3.7 15.8 1.0 OQ2 G:KCX169 3.8 14.8 1.0 CE1 G:HIS201 4.0 21.4 1.0 OD1 G:ASP301 4.1 13.1 1.0 ND1 G:HIS201 4.2 14.7 1.0 O G:HOH709 4.2 31.3 1.0 NE2 G:HIS57 4.3 11.4 1.0 OQ1 G:KCX169 4.3 12.9 1.0 CG G:ASP301 4.3 16.2 1.0 CX G:KCX169 4.5 14.5 1.0 NE1 G:TRP131 4.6 14.2 1.0 NE2 G:HIS230 4.6 13.2 1.0 CZ2 G:TRP131 4.6 16.7 1.0 NH1 G:ARG254 4.8 26.3 1.0 CE2 G:TRP131 4.9 17.6 1.0 CE1 G:HIS57 4.9 14.3 1.0

E.Campbell, M.Kaltenbach, G.J.Correy, P.D.Carr, B.T.Porebski, E.K.Livingstone, L.Afriat-Jurnou, A.M.Buckle, M.Weik, F.Hollfelder, N.Tokuriki, C.J.Jackson. The Role of Protein Dynamics in the Evolution of New Enzyme Function. Nat.Chem.Biol. V. 12 944 2016.
ISSN: ESSN 1552-4469
PubMed: 27618189
DOI: 10.1038/NCHEMBIO.2175