Arsenic in PDB 5hr7: X-Ray Crystal Structure of C118A Rlmn From Escherichia Coli with Cross-Linked in Vitro Transcribed Trna

All present enzymatic activity of X-Ray Crystal Structure of C118A Rlmn From Escherichia Coli with Cross-Linked in Vitro Transcribed Trna:
2.1.1.192;

The structure of X-Ray Crystal Structure of C118A Rlmn From Escherichia Coli with Cross-Linked in Vitro Transcribed Trna, PDB code: 5hr7 was solved by E.L.Schwalm, T.L.Grove, S.J.Booker, A.K.Boal, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	50.00 / 2.40
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	90.717, 70.383, 151.810, 90.00, 90.11, 90.00
R / Rfree (%)	21.6 / 24.8

The structure of X-Ray Crystal Structure of C118A Rlmn From Escherichia Coli with Cross-Linked in Vitro Transcribed Trna also contains other interesting chemical elements:

Magnesium	(Mg)	7 atoms
Iron	(Fe)	8 atoms

The binding sites of Arsenic atom in the X-Ray Crystal Structure of C118A Rlmn From Escherichia Coli with Cross-Linked in Vitro Transcribed Trna (pdb code 5hr7). This binding sites where shown within 5.0 Angstroms radius around Arsenic atom.
In total 2 binding sites of Arsenic where determined in the X-Ray Crystal Structure of C118A Rlmn From Escherichia Coli with Cross-Linked in Vitro Transcribed Trna, PDB code: 5hr7:
Jump to Arsenic binding site number: 1; 2;

Arsenic binding site 1 out of 2 in the X-Ray Crystal Structure of C118A Rlmn From Escherichia Coli with Cross-Linked in Vitro Transcribed Trna


Mono view


Stereo pair view

A full contact list of Arsenic with other atoms in the As binding site number 1 of X-Ray Crystal Structure of C118A Rlmn From Escherichia Coli with Cross-Linked in Vitro Transcribed Trna within 5.0Å range: probe atom residue distance (Å) B Occ B:As53 b:0.5 occ:1.00 AS B:CAS53 0.0 0.5 1.0 CE2 B:CAS53 2.0 95.5 1.0 CE1 B:CAS53 2.0 94.9 1.0 SG B:CAS53 2.3 83.9 1.0 CB B:CAS53 3.2 78.6 1.0 N B:CAS53 3.5 71.4 1.0 C B:TYR52 3.5 69.5 1.0 O B:HIS51 3.7 64.7 1.0 O B:TYR52 3.9 70.2 1.0 CA B:TYR52 3.9 68.5 1.0 CA B:CAS53 3.9 72.8 1.0 C B:HIS51 4.2 63.4 1.0 N B:TYR52 4.2 65.0 1.0 O B:TYR50 4.4 60.0 1.0 O B:ALA156 4.7 60.2 1.0 C B:ALA156 4.8 58.7 1.0 CB B:ALA156 4.8 54.2 1.0 CA B:LYS157 5.0 63.2 1.0

Arsenic binding site 2 out of 2 in the X-Ray Crystal Structure of C118A Rlmn From Escherichia Coli with Cross-Linked in Vitro Transcribed Trna


Mono view


Stereo pair view

A full contact list of Arsenic with other atoms in the As binding site number 2 of X-Ray Crystal Structure of C118A Rlmn From Escherichia Coli with Cross-Linked in Vitro Transcribed Trna within 5.0Å range: probe atom residue distance (Å) B Occ A:As53 b:0.2 occ:1.00 AS A:CAS53 0.0 0.2 1.0 CE2 A:CAS53 2.0 97.9 1.0 CE1 A:CAS53 2.0 96.7 1.0 SG A:CAS53 2.3 83.1 1.0 CB A:CAS53 3.2 77.4 1.0 N A:CAS53 3.5 70.4 1.0 C A:TYR52 3.5 67.6 1.0 O A:HIS51 3.8 66.8 1.0 O A:TYR52 3.8 67.0 1.0 CA A:CAS53 3.9 71.9 1.0 CA A:TYR52 4.0 68.3 1.0 C A:HIS51 4.2 64.7 1.0 N A:TYR52 4.3 66.0 1.0 O A:TYR50 4.4 61.2 1.0 O A:ALA156 4.6 60.1 1.0 C A:ALA156 4.8 58.7 1.0 CA A:LYS157 4.8 63.0 1.0 CB A:ALA156 4.9 54.3 1.0 N A:LYS157 4.9 60.6 1.0

E.L.Schwalm, T.L.Grove, S.J.Booker, A.K.Boal. Crystallographic Capture of A Radical S-Adenosylmethionine Enzyme in the Act of Modifying Trna. Science V. 352 309 2016.
ISSN: ESSN 1095-9203
PubMed: 27081063
DOI: 10.1126/SCIENCE.AAD5367