Arsenic in PDB 7cz0: Crystal Structure of A Thermostable Green Fluorescent Protein (Tgp) with A Synthetic Nanobody (SB92)

The structure of Crystal Structure of A Thermostable Green Fluorescent Protein (Tgp) with A Synthetic Nanobody (SB92), PDB code: 7cz0 was solved by H.Cai, H.Yao, T.Li, C.Hutter, Y.Tang, Y.Li, M.Seeger, D.Li, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	47.62 / 2.77
Space group	P 21 21 2
Cell size a, b, c (Å), α, β, γ (°)	95.245, 130.154, 171.453, 90, 90, 90
R / Rfree (%)	20.2 / 24.1

The binding sites of Arsenic atom in the Crystal Structure of A Thermostable Green Fluorescent Protein (Tgp) with A Synthetic Nanobody (SB92) (pdb code 7cz0). This binding sites where shown within 5.0 Angstroms radius around Arsenic atom.
In total 2 binding sites of Arsenic where determined in the Crystal Structure of A Thermostable Green Fluorescent Protein (Tgp) with A Synthetic Nanobody (SB92), PDB code: 7cz0:
Jump to Arsenic binding site number: 1; 2;

Arsenic binding site 1 out of 2 in the Crystal Structure of A Thermostable Green Fluorescent Protein (Tgp) with A Synthetic Nanobody (SB92)


Mono view


Stereo pair view

A full contact list of Arsenic with other atoms in the As binding site number 1 of Crystal Structure of A Thermostable Green Fluorescent Protein (Tgp) with A Synthetic Nanobody (SB92) within 5.0Å range: probe atom residue distance (Å) B Occ A:As304 b:351.5 occ:1.00 AS A:CAD304 0.0 351.5 1.0 O2 A:CAD304 1.6 61.4 1.0 O1 A:CAD304 1.8 83.2 1.0 C1 A:CAD304 2.0 89.8 1.0 C2 A:CAD304 2.0 118.1 1.0 CB A:PRO142 4.2 45.9 1.0 CB A:ASP193 4.3 34.3 1.0 O A:SER143 4.3 31.8 1.0 OD1 A:ASP193 4.4 48.4 1.0 CG A:ASP193 4.5 54.1 1.0 O A:HIS194 4.7 37.1 1.0 OG1 A:THR144 4.8 47.0 1.0 N A:SER143 4.9 36.0 1.0 CG A:PRO142 5.0 34.3 1.0

Arsenic binding site 2 out of 2 in the Crystal Structure of A Thermostable Green Fluorescent Protein (Tgp) with A Synthetic Nanobody (SB92)


Mono view


Stereo pair view

A full contact list of Arsenic with other atoms in the As binding site number 2 of Crystal Structure of A Thermostable Green Fluorescent Protein (Tgp) with A Synthetic Nanobody (SB92) within 5.0Å range: probe atom residue distance (Å) B Occ D:As301 b:157.8 occ:1.00 AS D:CAC301 0.0 157.8 1.0 O2 D:CAC301 1.7 64.6 1.0 O1 D:CAC301 1.7 48.7 1.0 C1 D:CAC301 2.0 63.6 1.0 C2 D:CAC301 2.0 87.2 1.0 O1 D:GOL302 3.9 60.1 1.0 OD2 D:ASP173 4.1 52.7 1.0 O B:VAL149 4.3 39.4 1.0 CG D:ASP173 4.3 61.2 1.0 OD1 D:ASP173 4.4 57.7 1.0 CB B:TYR148 4.6 41.8 1.0 N B:VAL149 4.6 35.7 1.0 CA D:GLU97 4.7 41.9 1.0 CB D:GLU97 4.7 40.9 1.0 CB B:ALA189 4.7 28.1 1.0 CB D:ARG171 4.8 37.5 1.0 O D:THR95 4.8 46.2 1.0 N D:GLU97 5.0 41.2 1.0

H.Cai, H.Yao, T.Li, C.Hutter, Y.Tang, Y.Li, M.Seeger, D.Li. An Improved Fluorescent Protein Tag and Its Nanobodies For Membrane Protein Expression, Stability Assay, and Purification To Be Published.