Arsenic in PDB 7v97: Arsenic-Bound P53 Dna-Binding Domain Mutant V272M

The structure of Arsenic-Bound P53 Dna-Binding Domain Mutant V272M, PDB code: 7v97 was solved by M.Lu, Y.F.Xing, Z.Y.Wang, Y.Ni, H.X.Song, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	42.80 / 2.02
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	68.839, 71.524, 84.684, 90, 90, 90
R / Rfree (%)	23.4 / 25.1

The structure of Arsenic-Bound P53 Dna-Binding Domain Mutant V272M also contains other interesting chemical elements:

Zinc

(Zn)

4 atoms

The binding sites of Arsenic atom in the Arsenic-Bound P53 Dna-Binding Domain Mutant V272M (pdb code 7v97). This binding sites where shown within 5.0 Angstroms radius around Arsenic atom.
In total 4 binding sites of Arsenic where determined in the Arsenic-Bound P53 Dna-Binding Domain Mutant V272M, PDB code: 7v97:
Jump to Arsenic binding site number: 1; 2; 3; 4;

Arsenic binding site 1 out of 4 in the Arsenic-Bound P53 Dna-Binding Domain Mutant V272M


Mono view


Stereo pair view

A full contact list of Arsenic with other atoms in the As binding site number 1 of Arsenic-Bound P53 Dna-Binding Domain Mutant V272M within 5.0Å range: probe atom residue distance (Å) B Occ A:As301 b:21.5 occ:0.40 SG A:CYS124 2.1 21.5 1.0 SG A:CYS141 2.1 21.2 1.0 SG A:CYS135 2.1 21.9 1.0 CB A:CYS135 2.7 20.7 1.0 CB A:CYS124 3.2 20.9 1.0 CB A:CYS141 3.2 19.7 1.0 CG A:MET133 3.3 21.9 1.0 CA A:CYS135 3.6 20.1 1.0 CA A:CYS141 3.7 18.8 1.0 SD A:MET133 3.8 24.2 1.0 CA A:CYS124 3.8 20.5 1.0 O A:PHE134 3.9 19.9 1.0 N A:CYS135 4.1 19.7 1.0 C A:PHE134 4.2 19.8 1.0 CE A:MET133 4.3 22.9 1.0 C A:CYS124 4.6 19.7 1.0 C A:CYS141 4.7 18.2 1.0 O A:THR140 4.7 18.1 1.0 N A:CYS141 4.7 18.5 1.0 N A:THR125 4.7 18.9 1.0 CB A:MET133 4.8 21.3 1.0 C A:CYS135 4.9 19.8 1.0 O A:THR123 5.0 21.9 1.0 SD A:MET272 5.0 21.5 1.0

Arsenic binding site 2 out of 4 in the Arsenic-Bound P53 Dna-Binding Domain Mutant V272M


Mono view


Stereo pair view

A full contact list of Arsenic with other atoms in the As binding site number 2 of Arsenic-Bound P53 Dna-Binding Domain Mutant V272M within 5.0Å range: probe atom residue distance (Å) B Occ B:As301 b:29.4 occ:0.40 SG B:CYS124 2.1 30.4 1.0 SG B:CYS141 2.1 30.0 1.0 SG B:CYS135 2.1 27.7 1.0 CB B:CYS135 2.7 26.1 1.0 CB B:CYS141 2.8 28.0 1.0 CB B:CYS124 3.3 29.3 1.0 CA B:CYS141 3.3 27.5 1.0 CG B:MET133 3.5 25.1 1.0 CA B:CYS135 3.7 25.5 1.0 SD B:MET133 3.9 27.4 1.0 O B:PHE134 4.0 24.4 1.0 CA B:CYS124 4.2 28.5 1.0 N B:CYS135 4.3 25.1 1.0 O B:THR140 4.3 25.5 1.0 C B:PHE134 4.4 24.6 1.0 N B:CYS141 4.4 27.0 1.0 C B:CYS141 4.4 27.1 1.0 CE B:MET133 4.5 26.5 1.0 C B:THR140 4.7 26.2 1.0 CB B:MET133 5.0 24.7 1.0

Arsenic binding site 3 out of 4 in the Arsenic-Bound P53 Dna-Binding Domain Mutant V272M


Mono view


Stereo pair view

A full contact list of Arsenic with other atoms in the As binding site number 3 of Arsenic-Bound P53 Dna-Binding Domain Mutant V272M within 5.0Å range: probe atom residue distance (Å) B Occ C:As301 b:29.2 occ:0.40 SG C:CYS124 2.1 31.8 1.0 SG C:CYS135 2.1 30.4 1.0 SG C:CYS141 2.2 26.2 1.0 CB C:CYS135 2.5 29.1 1.0 CB C:CYS141 3.0 24.4 1.0 CB C:CYS124 3.2 29.9 1.0 CA C:CYS135 3.4 28.6 1.0 CA C:CYS141 3.5 23.5 1.0 CG C:MET133 3.6 27.9 1.0 CA C:CYS124 4.0 28.8 1.0 N C:CYS135 4.0 27.6 1.0 O C:PHE134 4.1 27.0 1.0 SD C:MET133 4.1 30.2 1.0 C C:PHE134 4.2 26.5 1.0 N C:CYS141 4.6 24.1 1.0 C C:CYS141 4.6 22.4 1.0 O C:THR140 4.7 25.3 1.0 CE C:MET133 4.7 29.6 1.0 C C:CYS135 4.7 28.9 1.0 C C:CYS124 4.9 26.1 1.0 N C:THR125 4.9 23.8 1.0 C C:THR140 5.0 24.8 1.0

Arsenic binding site 4 out of 4 in the Arsenic-Bound P53 Dna-Binding Domain Mutant V272M


Mono view


Stereo pair view

A full contact list of Arsenic with other atoms in the As binding site number 4 of Arsenic-Bound P53 Dna-Binding Domain Mutant V272M within 5.0Å range: probe atom residue distance (Å) B Occ D:As301 b:23.3 occ:0.40 SG D:CYS124 2.1 23.5 1.0 SG D:CYS135 2.1 23.6 1.0 SG D:CYS141 2.1 22.8 1.0 CB D:CYS135 2.7 22.6 1.0 CB D:CYS141 3.0 21.5 1.0 CB D:CYS124 3.2 22.1 1.0 CA D:CYS141 3.4 20.8 1.0 CG D:MET133 3.5 23.7 1.0 SD D:MET133 3.6 24.0 1.0 CA D:CYS135 3.8 22.0 1.0 CA D:CYS124 3.9 21.3 1.0 O D:PHE134 4.1 20.9 1.0 CE D:MET133 4.2 23.4 1.0 C D:CYS141 4.4 20.4 1.0 O D:THR140 4.4 20.3 1.0 N D:CYS135 4.4 21.9 1.0 N D:CYS141 4.5 20.6 1.0 C D:PHE134 4.5 21.4 1.0 C D:CYS124 4.8 20.0 1.0 CD D:PRO142 4.8 19.9 1.0 N D:THR125 4.8 18.8 1.0 C D:THR140 4.8 20.6 1.0 N D:PRO142 4.9 20.2 1.0 O D:THR123 4.9 22.8 1.0

H.X.Song, J.L.Wu, Y.G.Tang, M.Lu. Landscape of Arsenic-Rescuable P53 Mutations Potentializes Personalized P53-Targeted Therapy To Be Published.