Arsenic in PDB 4nlw: Poliovirus Polymerase - G289A/C290I Loop Mutant

All present enzymatic activity of Poliovirus Polymerase - G289A/C290I Loop Mutant:
2.7.7.48;

The structure of Poliovirus Polymerase - G289A/C290I Loop Mutant, PDB code: 4nlw was solved by A.J.Sholders, O.B.Peersen, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	30.00 / 2.10
Space group	P 65
Cell size a, b, c (Å), α, β, γ (°)	128.845, 128.845, 111.733, 90.00, 90.00, 120.00
R / Rfree (%)	22.2 / 24.2

The structure of Poliovirus Polymerase - G289A/C290I Loop Mutant also contains other interesting chemical elements:

Sodium

(Na)

1 atom

The binding sites of Arsenic atom in the Poliovirus Polymerase - G289A/C290I Loop Mutant (pdb code 4nlw). This binding sites where shown within 5.0 Angstroms radius around Arsenic atom.
In total 2 binding sites of Arsenic where determined in the Poliovirus Polymerase - G289A/C290I Loop Mutant, PDB code: 4nlw:
Jump to Arsenic binding site number: 1; 2;

Arsenic binding site 1 out of 2 in the Poliovirus Polymerase - G289A/C290I Loop Mutant


Mono view


Stereo pair view

A full contact list of Arsenic with other atoms in the As binding site number 1 of Poliovirus Polymerase - G289A/C290I Loop Mutant within 5.0Å range: probe atom residue distance (Å) B Occ A:As96 b:54.1 occ:1.00 AS A:CAS96 0.0 54.1 1.0 CE2 A:CAS96 2.2 51.8 1.0 CE1 A:CAS96 2.2 51.8 1.0 SG A:CAS96 2.3 47.2 1.0 CB A:CAS96 3.1 42.5 1.0 OD2 A:ASP99 4.5 47.6 1.0 OD1 A:ASP99 4.6 46.8 1.0 O A:HOH1121 4.6 71.0 1.0 CG A:ASP99 4.6 45.2 1.0 CA A:CAS96 4.6 41.5 1.0 CB A:GLU98 4.8 42.7 1.0

Arsenic binding site 2 out of 2 in the Poliovirus Polymerase - G289A/C290I Loop Mutant


Mono view


Stereo pair view

A full contact list of Arsenic with other atoms in the As binding site number 2 of Poliovirus Polymerase - G289A/C290I Loop Mutant within 5.0Å range: probe atom residue distance (Å) B Occ A:As281 b:31.2 occ:1.00 AS A:CAS281 0.0 31.2 1.0 CE2 A:CAS281 2.2 31.5 1.0 CE1 A:CAS281 2.2 29.3 1.0 SG A:CAS281 2.2 27.9 1.0 CB A:CAS281 3.1 28.4 1.0 CB A:HIS272 3.7 29.5 1.0 CA A:CAS281 3.7 25.7 1.0 O A:SER271 3.9 24.4 1.0 C A:SER271 4.1 25.3 1.0 CA A:HIS272 4.2 27.9 1.0 N A:HIS272 4.2 26.2 1.0 CZ2 A:TRP5 4.4 24.5 1.0 CG A:HIS272 4.5 30.5 1.0 C A:CAS281 4.6 25.1 1.0 N A:VAL282 4.7 25.1 1.0 N A:CAS281 4.8 27.2 1.0 CA A:SER271 4.9 26.9 1.0 CD2 A:HIS270 5.0 33.3 1.0 N A:SER271 5.0 25.2 1.0 CE2 A:TRP5 5.0 23.4 1.0

A.J.Sholders, O.B.Peersen. Distinct Conformations of A Putative Translocation Element in Poliovirus Polymerase. J.Mol.Biol. V. 426 1407 2014.
ISSN: ISSN 0022-2836
PubMed: 24424421
DOI: 10.1016/J.JMB.2013.12.031